![]() ![]() To establish compatibility of donor and recipient for organ and bone marrow transplantation.Ĭurrently, HLA typing is performed in clinical laboratories by amplifying the genomic DNA encoding these genes and sequencing Determining the identities of HLA alleles that are present within an individual's genome (HLA typing) is of central importance Of antigens on the cell surface ( Shiina et al. HLA genes encode for the major histocompatibility complex (MHC) group of immune receptors which are instrumental in the presentation Second, full-length cDNA sequencing could be used to accurately determine the sequence and identity of alleles of HLA genes. Determining isoform-level transcriptomes of healthy and cancerous immune cells might inform treatment decisions and future However, evidence is accumulating that these epitopes might be absent in some isoforms expressed by these genes ( Sotillo et al. Leukemia (B-ALL) target epitopes of CD19, CD20, and CD22 ( Davila and Brentjens 2016 Fry et al. For example, current antibody and chimeric antigen receptor (CAR) T cell therapies against B cell acute lymphoblastic (1) the isoforms of surface receptors targeted in immunotherapy, (2) allele-resolved HLA transcript sequences central to self/non-self-discrimination,Īnd (3) B cell receptor (BCR) and T cell receptor (TCR) repertoires instrumental to the adaptive immune response to pathogens.įirst, full-length cDNA sequencing should be capable of investigating the transcript isoforms of surface receptors expressedīy B cells which have important roles in the immune response but are also themselves targets in the treatment of B cell–derived Accurate and deep full-length cDNA sequencing of immune cell transcriptomes could overcome this shortfall by providing However, RNA-seq, the current gold-standard for whole-transcriptome analysis, falls short of describing these immune receptorsĬompletely and accurately ( Mose et al. The transcriptsĮncoding these immune receptors are of great interest to basic and translational research as well as diagnostic and otherĬlinical purposes ( Logan et al. The human immune system relies on highly diverse and complex receptors to protect us from a wide array of pathogens. The HLA and AIRRĪnalysis approaches we introduce here are untargeted and therefore do not require prior knowledge of the composition or genetic Sequences of HLA alleles, and (3) extract detailed AIRR data for the analysis of the adaptive immune system. We used this data set to (1) show that deep and accurate full-lengthĬDNA sequencing can be used to provide isoform-level transcriptome analysis for more than 9000 loci, (2) generate accurate Generated over 10,000,000 full-length cDNA sequences at a median accuracy of 97.9% using our nanopore sequencing-based RollingĬircle Amplification to Concatemeric Consensus (R2C2) protocol. Sequencing (AIRR-seq) currently rely on our incomplete knowledge of the genetic diversity at HLA and BCR/TCR loci. ![]() Both short-read RNA-seq-based HLA typing and BCR/TCR repertoire Individual carry a vast amount of health-relevant information. ![]() In turn, the repertoires of millions of unique BCR and TCR transcripts in each In organ and bone marrow transplantations. Determining which allelesĪn individual possesses for each HLA gene (high-resolution HLA typing) is essential to establish donor–recipient compatibility These include transcriptsĮncoding human leukocyte antigen (HLA) receptors as well as B cell and T cell receptors (BCR and TCR). The human immune system relies on highly complex and diverse transcripts and the proteins they encode. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |